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This volume looks the current advanced protocols used to study aspects of the B cell receptor (BCR). The chapters in this book cover topics, such as the mutant of BCR repertoire to understand antibody evolution; interactions between B cells and viruses; mechanical force during BCR activation; B cell signaling using flow cytometry; confocal microscopy, total internal reflection microscopy and intravital two-photon microscopy; and the methods used to study critical cell components related with B cell activation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-e dge an d practical, B Cell Receptor Signaling: Methods and Protocols is a valuable resource for everyone in the scientific community currently working in the B cell field.
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