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Barley is one of the oldest cereals grown in the world, and is important for human and animal nutrition. The peripheral layers (PL), a co-product used for chemical protection of the endosperm and germ, are an important source of micronutrients and offer numerous health benefits. Little work has been done on the structural composition of developing barley grain tissues. The aim of this study is to determine the components of the isolated (CP) tissues originating during the filling phase of the barley grain (Saida 183), as well as the progressive changes undergone during this phase. Optical and scanning electron microscope observations were carried out to assess tissue purity. Ethanol maceration, physico-chemical analyses (minerals, crude fiber, phytic acid, proline and proteins)
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