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Ganoderma lucidum is one of the most important member among the Basidiomycetes. These fugal species have equal medicinal and therapeutical importance. They have exhibited various phenotypical features and response to other living organisms in a variable fashion. Recent study was plotted to examine the molecular identity among the species of Ganoderma lucidum colonizing at various sites in Lahore and also to evaluate their antimicrobial potential.