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This book constitutes the refereed proceedings of the First§International Conference on Computational Systems-Biology and§Bioinformatics, CSBio 2010, held in Bangkok, Thailand, in November 2010.§The 19 revised full papers presented were carefully reviewed and§selected from a total of 48 submissions. The papers cover a wide range§of topics and are organized in five topical sections on modeling and§simulation of biological processes, gene expression analysis, biological§sequence analysis and network reconstruction, bio-data visualization and§biological databases, as well as medical and biomedical informatics.