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Quantifying Protein Adsorption on Combinatorial Metal Films
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This book is a 2008 MSc thesis that presents new §high throughput techniques for quantifying protein §adsorption on metal surfaces. Using a materials §synthesis approach developed at Dalhousie §University, combinatorially sputtered Al-, Nb-, Ta-, §and Ti-containing films were prepared and §characterized. In order to test the metal surfaces §for their protein affinity ex-situ, a calibration §method was devised for wavelength-dispersive §spectroscopy. Fibrinogen and albumin absorption §amounts on each film were correlated to their §compositional gradient and were directly compared to §the adsorbed amounts obtained ex-situ using §spectroscopic ellipsometry. A simple and novel flow §cell design is also presented here for use with §variable angle spectroscopic ellipsometry to study §the adsorption of liquid-borne species in-situ. The §flow cell allows a sample to be probed point by §point and at any common ellipsometric angle of §incidence, unlike other designs.
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